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Hoof Wall Separation Disease (HWSD)


→ Detachment of the Hoof and Hoofwall.

Instructions for ordering:

  1. Enter the information of the first horse for which you want to order the test
  2. Add to cart
  3. Go to cart to check out, or
  4. Enter the information for a second horse
  5. For sample collection follow the instructions here.
  6. You can use this template to send in hair samples.
  7. Please also send the completed order form together with the samples.

For a breeder or veterinarian discount please contact us before ordering.

Any information you include here is optional, but will be included on the certificate of results (except “Additional info”).

Enter the name of your animal
Enter your animal's date/year of birth
Enter the breed name
Enter the registration number if known
Enter the microchip number
Is there anything else you would like to tell us about your horse? Reason for testing, symptoms etc.?

General Information

  • The first symptoms are commonly noticed within the first year.
  • As the hoofwall separates from the hoof, the horse can become lame.
  • Although some affected horses have minimal symptoms, the worst cases can require euthanasia because of severe pain.


  • Unstabile, brittle hoofwall
  • Inflammation of the hooves
  • Hoof pain

Inheritance and Genotypes

→ HWSD is an autosomal recessive hereditary disease, meaning that horses with only one copy of the genetic variant (N/hwsd) are clinically normal carriers while horses with two copies of the genetic variant (hwsd/hwsd) are affected.

Genotype: The horse is: Effects:
N/N normal. The horse does not have any copies of the genetic variant causative for HWSD and therefore cannot pass it on to any offspring.
N/hwsd a carrier. The horse is clinically normal. The genetic variant causative for HWSD will be passed on to its offspring with a probability of 50%.
hwsd/hwsd affected. The genetic variant causative for HWSD will be passed on to all offspring. All offspring will be carriers (N/hwsd).


  • Carriers may be bred to normal animals (N/hwsd x N/N) without any risk of producing affected offspring. The offspring should also be tested before breeding to determine if they are carriers or normal.
  • Breeding two carriers (N/hwsd x N/hwsd) is not recommended due to the possibility of 25% of the offspring being affected.
  • Affected animals (hwsd/hwsd) may only be paired with non-carriers (N/N) following a critical review of the breeding value of the animal.


Finno, C.J., Stevens, C., Young, A., Affolter, V., Joshi, N.A., Ramsay, S., Bannasch, D.L.: SERPINB11 Frameshift Variant Associated with Novel Hoof Specific Phenotype in Connemara Ponies. PLoS Genet 11:e1005122, 2015. Pubmed reference: 25875171. DOI: 10.1371/journal.pgen.1005122.

Further Information is available on the OMIA website: Online Mendelian Inheritance in Animals.

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